Regulatory officials use analytical variations (AVs) as a guide for issuing a violation of the label guarantee. The total variation surrounding an analytical analysis of a feed product and comparing it to a label consists of (1) variation associated with the formulation and mixing in producing a feed, (2) variation of particle sizes in feed ingredients, (3) potential in gradient settling during transport, (4) variation associated with sampling, (5) variation associated with mixing and grinding samples, and (6) variation during analysis in the laboratory. Most variations associated with sampling, mixing, grinding, and analysis in the laboratory are known as a result of many years of study to arrive at official methods. The AVs listed below allow for the inherent variability in sources that follow the official methods, with more weight given to the areas of analytical variation. The laboratory reported value of an analyte in a sample is reviewed with respect to the guarantee using the AVs below as a guide.

Note that the AVs listed for minerals do not apply to cat and dog complete foods formulated to meet AAFCO requirements. These complete pet foods must meet the appropriate AAFCO pet food profile. All profiles have minimum mineral requirements and some have maximums as well. In this situation, the tolerances are national standards set by AAFCO but these are further adjusted by the Division to include sample variation. Current tolerances updated in 2018 (12 KAR 2:021 Section 10).